Detection of sialic acid on cultured cells by binding of a lectin from Limax flavus.
نویسندگان
چکیده
(J. Am. Soc. Nephrol. 1992; 3:113-1 15) washed gently in PBS and solubibized in 1 mL/wehl of 0. 1 % sodium dodecyb sulfate (BioRad, Richmond, CA), and the radioactivity was estimated with a gamma counter (LKB-Bnomma, Sweden). To study the effects of fixation, the lectin binding was measured In cells fixed in 95% ethanol. Because the fixed cells could not be solubihizcd completely in sodium dodecyl sulfate, the cell layers were scraped by cotton tips and the tips were counted.
منابع مشابه
Sialic-acid-binding lectin from the slug Limax flavus--cloning, expression of the polypeptide, and tissue localization.
A cDNA library of Limax flavus was constructed and screened for sialic-acid-specific lectins. Complementary DNA clones were categorized into seven groups corresponding to closely related but different sequences. Group 1 clones contained an ORF encoding 199 amino acids including a sequence identical to the partial amino acid sequence obtained from the lectin protein. Within its 1074-bp 3' untran...
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عنوان ژورنال:
- Journal of the American Society of Nephrology : JASN
دوره 3 1 شماره
صفحات -
تاریخ انتشار 1992